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Related post: October 1, 1981 to September 30, 1982 TITLE OF PROJECT (80 characters or less) Structure and Function of the ADV Genome NAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER PROFESSIONAL PERSONNEL ENGAGED ON THE PROJECT PI: M. E. Bloom OTHER: C. F. Gar on L. W. Mayer Medical Officer Head, Research Microbiologist Expert (Microbiologist) LPVD NIAID EM NIAID L^ISF NIAID COOPERATING UNITS (if any) None lab/branch Laboratory of Persistent Viral Diseases, Hamilton, MT 59840 INSTITUTE AND LOCATION NIAID. NIH, Bethesda, W) 20205 TOTAL MANYEARS: 1.1 PROFESSIONAL: ,6_ .5 CHECK APPROPRIATE BOX(ES) n (a) HUMAN SUBJECTS D (al) MINORS D (a2) INTERVIEWS D (b) HUMAN TISSUES (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) The purpose of this project is to define the genome structure of the non - defective parvovirus , Aleutian disease virus (ADV). A double stranded intracellular form of ADV DNA analogous to replicative form DNA of other parvovirus has been isolated. This DNA has been mapped with restriction endonucleases. The restriction map differs completely from those reported for other parvoviruses. Three segments of the DNA have been molecularly cloned (0.05-0.15 map units, 0.15-0.56 map units and 0.56-0.86 map units) in bacterial systems. Plans are to clone the remaining DNA segments, and use these reagents to compare DNA from various isolates of ADV that differ in their virulence for mink. 28-11 PHS-6040 (Rev. 2-81) SMITHSONIAN SCIENCE INFORMATION EXCHANGE PROJECT NUMBER (Oo NOT use this space) U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES PUBLIC HEALTH SERVICE NOTICE OF INTRAHURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00086-05 LPVD PERIOD COVERED October 1, 1981 to September 30, 1982 TITLE OF Caverta Price India PROJECT (80 characters or less) Mechanisms of Resistance to Graft Versus Host Disease NAMES, LABORATORY AND INSTITUTE AFFILIATIONS, AND TITLES OF PRINCIPAL INVESTIGATORS AND ALL OTHER PROFESSIONAL PERSONNEL ENGAGED ON THE PROJECT PI: J. L. Portia OTHER: M. W. Cloyd Medical Officer Senior Staff Fellow LPVD NIAID LPVD NIAID COOPERATING UNITS (if any) None lab/branch Laboratory of Persistent Viral Diseases, Hamilton, MT 59840 SECTION INSTITUTE AND LOCATION NTATD , N TH. Bethesda, M P 20205 TOTAL MANYEARS: 1.0 PROFESSIONAL: .5 OTHER: .5 CHECK APPROPRIATE BOX(ES) D (a) HUMAN SUBJECTS D(al) MINORS D {a2) INTERVIEWS n (b) HUMAN TISSUES gl (c) NEITHER SUMMARY OF WORK (200 words or less - underline keywords) Acute graft versus host disease (GVH) can d (B6 X DBA/2)F 1 Induced in unirradiated adult by the inoculation IV of 60-100 X 10 B6 spleen cells. GVH is not seen, however, in F^ recipients of as many as Caverta Uk 200 X 10 DBA/2 spleen cells, This resistance to acute GVH is abrogated by irradiation of the recipient prior to donor cell inoculation suggesting that the recipient participates in
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